Structure of the fucose mutarotase from Streptococcus pneumoniae in complex with L-fucose
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چکیده
منابع مشابه
The Metabolism of L - Fucose
The isomerization of L-fucose to L-fuculose was observed by Green and Cohen (2) in extracts obtained from Escherichia coli grown on L-fucose. With the same organism and similar conditions, Huang and Miller (3) suggested that such extracts catalyzed the phosphorylation of both L-fucose and L-fuculose to form the corresponding sugar l-phosphates. The present report describes the partial purificat...
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Human GDP-l-fucose synthase, also known as FX protein, synthesizes GDP-l-fucose from its substrate GDP-4-keto-6-deoxy-d-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4. In this paper, the first crystal structure of human FX protein was determined at 2.37 Å resolution. The asymmetric unit of the crystal structure co...
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Monomeric sugars generated during the metabolism of polysaccharides, glycoproteins, and glycolipids are imported to the cytoplasm and converted to respective nucleotide sugars via monosaccharide 1-phosphates, to be reutilized as activated sugars. Because L-fucose (L-Fuc) is activated mainly in the form of GDP derivatives in seed plants, the salvage reactions for L-Fuc are expected to be indepen...
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The first committed enzyme in GDP-L-fucose formation from GDP-D-mannose is GDP-D-mannose 4,6-dehydratase, which forms GDP-4-keto-6-deoxy-D-mannose. The uncertain enzymatic steps beyond this point were examined in this study. Assays were developed for the epimerase and reductase activities which the putative pathway would predict. A protein was isolated exhibiting homogeneity by several criteria...
متن کاملMetabolite Control of L - Fucose Utilization 8361
Thyroid fucokinase is responsive to a number of metabolites which might serve in a regulatory capacity. In addition to inhibition by ADP and stimulation by GMP, fucokinase responds selectively to a series of nucleotide sugars. Of those studied, only guanine nucleotide sugars moderate the activity of the enzyme. GDP-a-D-mannose, GDP-a-D-glucose, GDP-cY-D-rhamnose, and GDP-cu-L-rhamnose all stimu...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2011
ISSN: 1744-3091
DOI: 10.1107/s1744309111046343